DNA degradation in PFGE

Yves Bertheau bertheau at inapv.inapg.inra.fr
Wed Sep 3 12:56:38 EST 1997


Hello,

We started PFGE some months ago and we are in troubles with some
bacterial strains whose DNA is fully degraded during - or before ? -
the electrophoresis.

I was looking with Medline for papers about these troubles which appear
more frequent than I thought. However, I did not find a protocol
to suppress the degradation.

Does anyone have such a protocol or advices to eliminate this unexpected
DNA degradation ?

Thanks in advance for your help.

Yves Bertheau



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