Non-isotopic cDNA label?

[Anne Schmitz]

Dr. Peter Hovland wrote:
> 
> We are interested in creating cDNA probes to study gene expression.
> Does anyone have experience using non-radioactive substrates for reverse
> transcriptase?
> I thank you in advance,
> Dr. Peter Hovland
> Department of Radiology
> University of Colorado Health Sciences Center

I routinely use a 5'-biotinylated primer for primer extension. In my
hands, it works just fine. 

Regards, Anne


Anne Schmitz
University of Bielefeld
Department of Microbiology/Genetecnology