Anti-His6 antibodies

zakaria at ita.fhg.de zakaria at ita.fhg.de
Thu Sep 4 07:34:15 EST 1997


In article <5t8v8p$idj$1 at news.utu.fi>, heihei at utu.fi wrote:

> Hi there,
> Please give comments on any commercial anti-His6 antibodies you have
> tried, esp. Qiagene's Mabs: Which brand works best (in western)?  Do
> they generally work as well as promised? Any tips on sample
> formulation etc. 
> We are making secreted dimeric protein with N-term 6xHis in Pichia.
> Please reply at least on E-mail; I'm an infrequent reader so sorry if
> this is an old bone already.
> Cheers,
> Jussi Heimo


I try the antibody ANTI XPRESS His TAG against the 6 His from
Invitrogen.     But this antibody isn´t very specific. This ANtib. is
only  for a western after the last purification step

-- 
Fraunhofer Institut
Dep. Genetechnology
Nikolai-Fuchs-Str. 1
30625 Hannover
Germany



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