metabolic labeling in e.coli...

Frederik Boernke boernke at IPK-Gatersleben.de
Fri Sep 5 01:40:47 EST 1997


Hi!

Why don't you try 35S-methionine or cysteine (or comercially available
mixture of both; Amersham/NEN). For a 20 ml induction culture I usually
add 2 mCi of 35S-methionine 15 min post induction and incubate for 4 h.
I tried this with the TrxFus-System by Invitrogen and it worked fine but
you need quiete a few methionine residues in your protein (about 7) to
obtain sufficient specific acitivity.

Good luck
Ricky


******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de
http://www.ipk-gatersleben.de




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