Hsp92II - ? ligase activity; NlaIII - ?different grades

Dr. Duncan Clark duncan at genesys.demon.co.uk
Sat Sep 6 08:54:47 EST 1997

In article <5uorh7$apa$1 at proxy5.proxy.ozemail.net>, Ian & Viive Howell
<ihowell at ozemail.com.au> writes
>New England Biolabs (NlaIII) and Promega (Hsp92II) - AUD$82/500 U;
>Sigma and Amersham (NlaIII)  ~ AUD $269/ 20 U.
>The companies cound not explain the > 1000 fold price difference.  Are
>there 2 different grades of this enzyme??

NEB are the producer of Nla II from their proprietary patented clone. I
would think that the Sigma and Amersham produc is native enzyme and
therefore way more expensive as the strain produces 4 RE's and this
means extra purification etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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