exaggerated transformation efficiencies ?
s535290 at aix1.uottawa.ca
Mon Sep 8 04:15:27 EST 1997
I have now encountered two occasions where commercially available
"super-competent" cells have turned out to be no better than home made
competent cells I have made using the one step TSS protocol. What in the
heck is up with that ? have any of you found cells that are as competent
as advertised ? I do need very high efficiency for what I am doing, but
this is starting to make me a little peeved. I mean, a 100 fold
difference in efficiency ? and this is using ccc pUC18....
thanks for listening,
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