How to remove DNA from RNA for RT-PCR

coetzeew at is2.nyu.edu coetzeew at is2.nyu.edu
Mon Sep 8 11:26:49 EST 1997


See: BioTechniques Vol 22 No 6 (1997) p. 1128. 

     William Coetzee

On Wed, 03 Sep 1997 17:09:44 -0500, Marcos Malumbres
<malumm01 at mcrcr.med.nyu.edu> wrote:

>I've treated my RNA with RNase-free DNase I (following Sambrok et al.,
>and Ausubel et al. procedures) at least 3 consecutive times and I still
>get DNA-generated bands in my RT-PCR. Does anybody know a method to
>absolutely remove DNA contamination from RNA preparations?
>--
>
>******************************************************
>
>Marcos Malumbres
>
>Dep. of Pathology, New York University Medical Center
>550 First Ave. New York, NY10016
>Tel. (212)2635342, Fax (212)2638211
>------------------------------------------------------
>email:malumm01 at mcrcr.med.nyu.edu
>
>******************************************************
>
>




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