Brent/LexA 2-hybrid question about positives
polliwog at u.washington.edu
Tue Sep 9 02:07:57 EST 1997
What to make of a 2hybrid positive that is bot only Leu+ and
LacZ positives on galactose but is also quite blue on GLUCOSE?
Is the GAL1 promoter leaky? Is what I am seeing a detection of a very
strong interaction from a leaky cDNA transcription on glucose medium?
Seth D. Findley polliwog at u.washington.edu
Department of Biochemistry Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195
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