Brent/LexA 2-hybrid question about positives

S. Findley polliwog at
Tue Sep 9 02:07:57 EST 1997

What to make of a 2hybrid positive that is bot only Leu+ and 
LacZ positives on galactose but is also quite blue on GLUCOSE?

Is the GAL1 promoter leaky? Is what I am seeing a detection of a very
strong interaction from a leaky cDNA transcription on glucose medium?


Seth D. Findley               polliwog at
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195

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