Chloroplast isolation

Dr. Peter Gegenheimer PGegen at UKans.edu
Tue Sep 9 16:16:18 EST 1997


On Wed, 3 Sep 1997 12:40:22, rabc010 at S1.CXWMS.AC.UK wrote:

> 
> There are decent protocols for isolation of chloroplast and mito-
> chondria in Methods in Enzymol. vol. 97. and in Plant Molecular ......
   
The Methods in Enzymology references are Meth. Enzymol. vol. 118 
(1986) (Plant Molecular Biology) and Meth Enzymol vol. 182, 174-193 
(1990) (Protein Purification). The latter article has a typo: 
Percoll gradients are made with 1.2 vol 40% and 1.0 vol 80% Percoll 
mixes. Several articles in vol 118 discuss preparation of pure 
chloroplast DNA. 

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