Two questions about southern
peng.36 at postbox.acs.ohio-state.edu
Tue Sep 9 12:41:57 EST 1997
I amplified more than ten sequences after DNA subtractive hybridization.
These sequences were supposed present in one genomic DNA sample ( tracer) but
absent in another (Driver). To confirm it, I am planning do sorthern blot.
Now two questions come out:
1. Could I use the genomic DNA as a probe to probe the amplified sequences,
in stead of using the sequences to probe the genomic DNA? The genomic DNA is
about a hundred times less complicate than human genome.
2. What is the best processure of non-radioactive southern? p32 works fine
for me. But I have to switch to non-radioactive labeling.
Any advices are welcomed.
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