Lane-tracking and non-ABI sequencers

crepinau at sb-roscoff.fr crepinau at sb-roscoff.fr
Thu Sep 11 09:23:29 EST 1997


In article
<341433D4.450
0 at chugaibio.c
om>,

dspinella at chu
gaibio.com,
@chugaibio.co
m wrote:
> I think that if you investigate
> carefully, you will also find that other brands of automated Sequencers
> suffer from different (and more insidious) problems.  The most severe
> such problem is that the base-calling algorithms are not as good as with
> ABI and there are more mis-calls.

I don't agree
with you, I
am a user of
the Vistra
system and
very happy
with
the base
calling
software. The
study that
was done on
sequencing
facilities
(it is
available on
the WWW but I
can't recall
the
address)
shows that
accuracy
is not linked
with a brand,
as the very
same
automatic
sequencer
could be in
the top 10 as
well as
beeing ranked
very low.
What
seems more
important in
the accuracy
of the
sequences
seams to be
the length
of the
migration.

>> and I sure hope they get it figured out. In the meantime, one solution
> (other than loading just half the lanes)

36 lanes
divided by 2
makes 18, I
don't know
how long
take a run to
complete
on a ABI, but
with a well
trained
technician
one can
makes 22
samples per
day
on a vistra.

I am not
affiliated to
Vistra, but
in my
opinion, it
is a good
sequencer for
small
sequencing
program, very
easy to use.
I don't have
any
problem of
lane tracking
(except when
the sequence
is very very
bad) no blue
blob or red
thingy. I
don't
have to spend
half an hour
washing the
plate because
there is
building up
(I
just use
Decon, a
classic
detergent,
rinse
 with
ddwater and
ethanol
before
pouring the
gel) and, at
least in
Europe, the
assistance is
very friendly
and
most of the
time
efficient.
There are
problems, the
worst beeing,
it is very
hard to do
more than 22
samples per
day, the
plates
beeing small,
it is hard
to read more
than 700 bp,
and there is
no dye
termination
kit at the
minute.

I hope this
will help
you.

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet



More information about the Methods mailing list