Tunnel assay

David Carpio dcarpio at mail1.sas.upenn.edu
Thu Sep 11 09:51:04 EST 1997


I'm experiencing really fuzzy background staining with this assay.  I'm
using it on spinal cord and brain sections.  Although Tunnel positive
cells are clear enough, for photography purposes the background is to
high.  I was wondering if anyone with experience using Tunnel could give
me some suggestions how to reduce the background staining.

Thanks in advance,

Dave



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