How to deglycosylate a protein?

Thorsten Schmidt Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Thu Sep 11 15:58:41 EST 1997


Dear reader!

I examine a protein which I can detect by Western Blots.
It is detected at a higher molecular wight than expected according to
the aminoacid sequence.

I now want to test whether it is glycosylated or not!

How can I do this? What do I need?
Are there enzymes/kits availible for deglycosylation?

Are there different types of protein glycolysation and can I examine 
them all just with one test?

Can I conclude protein glycosylation just from special aminoacid sequences?

It would be too kind if you´ll send me an answer

Thank you so much in advance

Thorsten Schmidt





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