RNA isolation from cornea
GKidd at Aptagen.com
Thu Sep 11 18:23:26 EST 1997
Have you tried guanidium chloride? If I understand the structure of
the cornea correctly, it would seem that shredding wouldn't be necessary
with this method, since it would lyse the cells on the inner surface of
the cornea. Then you just spin down the debris and process the supe.
Roxanne Walder wrote:
> I would like to know if anyone has had great success in isolating RNA from
> cornea, preferably from human tissue. I have tried numerous protocols and
> kept getting very low yields. The tissue doesn't even shred with a
> Polytron. Please post your reply either on this page, or e-mail me
> directly at roxanne-walder at uiowa.edu. Thanks.
More information about the Methods