RNA isolation from cornea

[Geoffrey Kidd]

Have you tried guanidium chloride?  If I understand the structure of 
the cornea correctly, it would seem that shredding wouldn't be necessary 
with this method, since it would lyse the cells on the inner surface of 
the cornea.  Then you just spin down the debris and process the supe.

Geoffrey Kidd

Roxanne Walder wrote:
> 
> I would like to know if anyone has had great success in isolating RNA from
> cornea, preferably from human tissue.  I have tried numerous protocols and
> kept getting very low yields.  The tissue doesn't even shred with a
> Polytron.  Please post your reply either on this page, or e-mail me
> directly at roxanne-walder at uiowa.edu.  Thanks.