Strange contaminant in cell culture
Richard P Grant
rgrant at see.sig.for.address
Fri Sep 12 05:26:35 EST 1997
(Didn't see the original).
Tom Vink (tvink at xs4all.nl) wrote:
} 9321531k at clinmed.gla.ac.uk (Obaid Y. Khan) wrote:
} >We have been having some problems with our tissue
} >which might be a contamination.
} >What we are experiencing is round specks or particles
Does it go away when you filter your FCS?
Brownian motion can make debris look as if it's ... alive!
} A DNA staining of the particles could be helpful to prove if it is an
} organism or just debris.
Although sometimes debris can give a false +ve for (eg) mycoplasma tests.
Richard P. Grant MA DPhil University of Oxford | rgrant at molbiol
http://www.molbiol.ox.ac.uk/~rgrant FFPGP | ox ac uk
------# 'At this time we are currently boarding...' - heard at JFK #------
More information about the Methods