Strange contaminant in cell culture

Richard P Grant rgrant at see.sig.for.address
Fri Sep 12 05:26:35 EST 1997


(Didn't see the original).

Tom Vink (tvink at xs4all.nl) wrote:
} 9321531k at clinmed.gla.ac.uk (Obaid Y. Khan) wrote:

} >Hi

} >We have been having some problems with our tissue 
} >culture 
} >which might be a contamination. 
} >What we are experiencing is round specks or particles 

....


Does it go away when you filter your FCS?

Brownian motion can make debris look as if it's ... alive!


R

} A DNA staining of the particles could be helpful to prove if it is an
} organism or just debris.

Although sometimes debris can give a false +ve for (eg) mycoplasma tests.

--
Richard P. Grant  MA  DPhil          University of Oxford | rgrant at molbiol
http://www.molbiol.ox.ac.uk/~rgrant  FFPGP                |    ox ac uk
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