FLAG affinity columns

D. Pearton pearton at saul7.u.washington.edu
Fri Sep 12 18:08:10 EST 1997


Hi all,

I'd like to solicit opinions from people who have used anti-FLAG affinity
gels to purify FLAG-tagged proteins.  I've used a M2 gel from IBI but I am
not happy with the performance of the M2 or M5 antibodies.  I know that
SantaCruz biotech has a good polyclonal anti-FLAG antibody and have had
fairly good results with this on blots and immunoflourescence.  Does
anyone know if they have this coupled to a gel, and, if so, is it a good
affinity reagent?

Thanks,

--
Dave Pearton
pearton at u.washington.edu
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Down he sank in a chair-ran his hands through his hair-	+
     And chanted in mimsiest tones			+
Words whose utter inanity proved his insanity,		+
     While he rattled a couple of bones.		+
							+
"The Hunting of the Snark" Lewis Carroll		+
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