In vitro phosphorylation of a peptide

Phillip Robinson phrobins at
Fri Sep 12 01:09:32 EST 1997

> We are planning to in vitro phosphorylate a 10 amino acid peptide
> containing an kinase recognition sequence (RRASV)?
> The question is: is this peptide to short and what would be the minimal
> length required for efficient phosphorylation?

10 amino acids is great.  I've gone down to 6, but it might well depend
on the kinase involved.  Obviously you are talking about PKA for this

Children's Medical Research Institute      ,
      Sydney, NSW AUSTRALIA            ;--_|\
                                      /      \
                                     (       *<-
    phrobins at         \_;--._/

More information about the Methods mailing list