thick, denaturing 1.5 mm gels?

Thomas urbig at biokemi.su.se
Tue Sep 16 10:59:23 EST 1997


In article <wad1-1209972059510001 at 132.236.171.141>, wad1 at cornell.edu
(Wayne Decatur) wrote:

> I am having trouble drying thick denaturing polyacrylamide gels.  If I run
> a long,thin 8% gel for seqeuncing drying works fine, but if I use a
> relatively thick (1.5 mm) gel it cracks during and after drying.  Is there
> something I can soak it in to eliminate this problem?  Do I need a
> different backing?  Our pump pulls the best it can and I don't have
> probles drying the same thickness of denaturing 5% gel.  Any ideas? 
> Please e-mail me directly at wad1 at cornell.edu and post it to the list if
> you think it would be of general interest.
> 
> Thanks for listening,
> Wayne Decatur
> Section of Biochemistry, Molecular and Cell Biology
> Cornell University
> Ithaca, NY 14853

Wayne, 

I soak the 1.5 mm thick SDS-PAGE (6-20% T) I run for 30mins in 30%
Methanol/3% Glycerol. After that I just put them on Whatman 3M paper, put
Saran on top and dry them for 45 mins, works every time- no trouble so
far.

Hope that helps

Thomas



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