thick, denaturing 1.5 mm gels?
urbig at biokemi.su.se
Tue Sep 16 10:59:23 EST 1997
In article <wad1-1209972059510001 at 18.104.22.168>, wad1 at cornell.edu
(Wayne Decatur) wrote:
> I am having trouble drying thick denaturing polyacrylamide gels. If I run
> a long,thin 8% gel for seqeuncing drying works fine, but if I use a
> relatively thick (1.5 mm) gel it cracks during and after drying. Is there
> something I can soak it in to eliminate this problem? Do I need a
> different backing? Our pump pulls the best it can and I don't have
> probles drying the same thickness of denaturing 5% gel. Any ideas?
> Please e-mail me directly at wad1 at cornell.edu and post it to the list if
> you think it would be of general interest.
> Thanks for listening,
> Wayne Decatur
> Section of Biochemistry, Molecular and Cell Biology
> Cornell University
> Ithaca, NY 14853
I soak the 1.5 mm thick SDS-PAGE (6-20% T) I run for 30mins in 30%
Methanol/3% Glycerol. After that I just put them on Whatman 3M paper, put
Saran on top and dry them for 45 mins, works every time- no trouble so
Hope that helps
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