pickup protein from conditioned DMEM

liua02 at mcrcr6.med.nyu.edu liua02 at mcrcr6.med.nyu.edu
Wed Sep 17 09:44:25 EST 1997


Hello,

I am trying to express Shh in mammalian cells and have detected the
RNA by northern. Now I want to know whether the secreted protein exist
in the feeding medium. Can anybody tell me how can I pickup proteins
from conditioned medium ( I mean, is there a simple way to concentrate
or precipitate the proteins from the medium? If some special reagent
or columns are to be used, can you suggest where I can buy them and
what is the catalogue number? Thanks a lot

Aimin



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