serum-free culture of CHO cells

harald at mol.univie.ac.at harald at mol.univie.ac.at
Wed Sep 17 12:45:10 EST 1997


Hi, netters!

I am looking for a media formulation suitable (or even specially designed)
for growing chinese hamster ovary (cho) cells. Does anybody have 
experience with these media, and doea anybody have a recipe?

If there are only commercially available media, these must be free of 
transferrin or other iron sources as well, since we need a defined iron 
content of the medium (we add Tf later, anyway, but the concentration is 
critical).

Thanks for any suggestions,

Harald




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