filter-sterilized MilliQ water for PCR?

[Jun-Ichi Aikawa]

Clontech recommended to use filter-sterilized MilliQ water for PCR instead
of autoclaved water, because autoclave can introduce salts. Does anyone use
filter-sterilized water for PCR? Do I need to worry about its nuclease or
protease contamination?

Jun-ichi Aikawa, Ph.D.
Division of Cellular and Molecular Medicine
University of California, San Diego