filter-sterilized MilliQ water for PCR?

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Wed Sep 17 03:24:16 EST 1997

Clontech recommended to use filter-sterilized MilliQ water for PCR instead
of autoclaved water, because autoclave can introduce salts. Does anyone use
filter-sterilized water for PCR? Do I need to worry about its nuclease or
protease contamination?

Jun-ichi Aikawa, Ph.D.
Division of Cellular and Molecular Medicine
University of California, San Diego

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