Best vector for cloning of Taq-generated PCR products

Tom Vink tvink at xs4all.nl
Thu Sep 18 05:48:30 EST 1997


On Fri, 12 Sep 1997 06:20:39 -0700, Michael Lassner
<mikel51 at mother.com> wrote:

>I have been using the new TopoTA vector from Invitrogen.  It is very
>fast and convenient, and it has been working well in my hands.  You have
>to thaw your competent cells before you start because you only incubate
>your PCR product with the vector for 5 minutes.  No enzymes, you just
>mix the vector and PCR product, let it sit at room temperature for 5
>minutes and then transform.  It is expensive so it is worth it to use a
>very good pipetor to dispense the 1 microliter of vector used for each
>cloning.  I am not associated with Invitrogen, except as a customer.
:
I agree. In our hands the topo kit works very efficiently much better
than other T-cloning kits.





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