Question about Immunoprecipitation

Basem M. William, MD william at WELCHLINK.WELCH.JHU.EDU
Thu Sep 18 13:19:41 EST 1997


Hi, everyone:

I am planning to IP a nuclear protein. However, general IP protocol
suggest to lyse the cell, follow by a low speed spin and use the
supernatant for IP.

My question is:
1) After the lysis and the low speed-spin, is the supernatant contains
any nuclear protein? (The pellet generated by low-speed spin should be
mostly nucleus, right?)
2) Is there a way to retrieve as much nuclear protein as possible
through the lysis step?

Thanks in advance.

Jining
jnbai at welchlink.welch.jhu.edu



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