dam negative e. coli

Dom Spinella dspinella at chugaibio.com
Thu Sep 18 09:21:33 EST 1997


> hi: i am looking for a dam methylase negative e. coli strain other
> than JM110. any clues?
> 
> thanx, bip
> 
> Bipin K. Dalmia, Ph.D.
> Protein Expression and Purification
> Pioneer Hi-Bred International, Inc.
> Johnston, Iowa 50131

Stratagene sells another strain called SCS110 which is both dam and dcm
negative.  Like JM110 however, its transformation efficiency is
miserable (~5e6 transformant per ug supercoiled plasmid).  Hope that
helps.  -- D.G. Spinella



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