Plasmid concentration

Frederik Boernke boernke at IPK-Gatersleben.de
Fri Sep 19 03:18:18 EST 1997


> Plasmid concentration
> 
> Cristina Cebrian (cebrian at ar.vhebron.es)
> Thu, 18 Sep 1997 19:10:41 GMT
> 
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> ----------------------------------------------------------------------

> 
> Just wandering... Is it possible to know plasmid DNA concentration
> without
> digestion? Is the 260 absorvance reliable? is it better to run a gel?
> 
> Thanks a lot!
> ----------------------------------------------------------------------
> 
>    * Next message: ellenfo at fagmed.uit.no: "PCR - Acanthamoeba
>      polyphaga"
>    * Previous message: Todd Serulneck: "human EGF"

Hi!

It depends on the method for isolation you employed. When you did 
alkaline lyses the sample will be to salty for measuring OD260, so you
should better run a gel and compare with a standard. When you did some 
kind of CsCl-gradient you can easily rely on the OD600.

Ricky



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