Alexander N. Kukushkin
kan at mmcd.cyt.ras.spb.ru
Fri Sep 19 09:12:34 EST 1997
> I'm trying to run a 1% agarose gel to separate RNA. I'm using a MOPS;
> EDTA; and Sodium Acetate Buffer with Formaldehyde. Following running at
> 100 Volts for an hour, I'm finding the (8 cm) gels breaking up with very
> little manipulation. Should I cool it in a fridge following the run?
> Any ideas-thanks dave.
After the run, wash the gel in water for 10-15 min to remove formaldehyde.
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