Fragile Agarose

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Fri Sep 19 09:12:34 EST 1997


>   I'm trying to run a 1% agarose gel to separate RNA.  I'm using a MOPS;
>   EDTA; and Sodium Acetate Buffer with Formaldehyde.  Following running at
>   100 Volts for an hour, I'm finding the (8 cm) gels breaking up with very
>   little manipulation.  Should I cool it in a fridge following the run?
>   Any ideas-thanks dave.
>
After the run, wash the gel in water for 10-15 min to remove formaldehyde.
Alex



More information about the Methods mailing list