Ni-agarose elution

brett brett at BORCIM.WUSTL.EDU
Sat Sep 20 11:02:33 EST 1997


>Hi
>I am on my way to purify a 6xHis tagged protein.  Does anybody know if I 
>can elute the protein from Ni-agarose by just adding SDS-PAGE loading 
>buffer, instead of using imidazole buffer?
>
>nam
>
>-- 
>***************************************************
>Tsunehisa Namba
>Department of Anesthesia, Kyoto University Hospital
>Sakyo-ku, Kyoto 606-01, Japan
>Fax:+81-75-752-3259
>E-mail: drnamba at kuhp.kyoto-u.ac.jp


I take it you're not interested in saving the resin.  Why not make your sample
buffer 100mM EDTA? That seems to work for me.
Brett Lindenbach
brett at borcim.wustl.edu

Dept Molecular Microbiology
Washington University School of Medicine
Box 8230
660 S. Euclid Ave
St Louis, MO 63110




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