Q. Monocytes isolation: Looking for protocol

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Mon Sep 22 05:59:49 EST 1997


I am looking for the possibility to isolate Monocytes. At the moment
I am working with a system that is called Larex Cellsep .
The problem lies therein, that Lymphocytes have a density between 
1.052 and 1,077g/ml. Monocytes have a desity between 1.05 and
1.066 g/ml .But this system is working with a density gradient media 
and so it will never be possible to isolate Monocytes by the 
Lymphocytes with this system.   
So I am looking for a not to difficult possibility to isolate 
Monocytes.

Tilo



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