Ni-agarose elution

Cornelius Krasel krasel at
Mon Sep 22 04:22:28 EST 1997

Charles A Miller (oravaxcm at wrote:
> One question, if you elute with EDTA (which will strip the
> nickel and protein complex off your column) will you still have
> nickel complexed with protein? Well, I guess not, it'll just chelate
> the nickel...which could get pretty expensive.

Why? Can't you recharge your column with a NiCl2 solution?


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */

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