Ni-agarose elution

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon Sep 22 04:22:28 EST 1997


Charles A Miller (oravaxcm at world.std.com) wrote:
> One question, if you elute with EDTA (which will strip the
> nickel and protein complex off your column) will you still have
> nickel complexed with protein? Well, I guess not, it'll just chelate
> the nickel...which could get pretty expensive.

Why? Can't you recharge your column with a NiCl2 solution?

--Cornelius.

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/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
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