Ni-agarose elution

[Cornelius Krasel]

Charles A Miller (oravaxcm at world.std.com) wrote:
> One question, if you elute with EDTA (which will strip the
> nickel and protein complex off your column) will you still have
> nickel complexed with protein? Well, I guess not, it'll just chelate
> the nickel...which could get pretty expensive.

Why? Can't you recharge your column with a NiCl2 solution?

--Cornelius.

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