RBS to ATG distance?

[Marieke R. Koedood Zhao]

Should work.

Marieke


Fourie Joubert (fourie at scientia.up.ac.za) wrote:
: Hi

: I'm cloning in a pET vector using the T7 promoter. As I understand it,
: the ribosomal binding site should occur 4-7 bases before the ATG start
: codon. 

: Will the protein still express if the ATG is 10-11 bases downstream 
: of the RBS?

: Any info would be sincerely be appreciated!

: __________________________________________________________________________

:      _/_/_/_/  _/_/_/_/_/  Fourie Joubert           
:     _/            _/     Department of Biochemistry
:    _/            _/    University of Pretoria
:   _/_/_/_/      _/   South Africa
:  _/            _/  fourie at scientia.up.ac.za
: _/      _/_/_/_/  http://suntiger.ee.up.ac.za/biodocs/home.html
:                  http://crimson.up.ac.za/~fourie
: __________________________________________________________________________