GST-fusion proteins

[Laboratory of Dr. Kevin D. Sarge]

Tina Myers wrote:
> 
> Hello,
> 
> Does anyone have any information regarding fusion of the GST gene to
> the COOH-terminus of a protein, i.e., can direct fusion be done or is it n
> necessary to have a spacer?  Thank you for your input.
> 
> tina at borcim.wustl.edu


It's been my experience that it varies by protein.  If the C-terminus of
your protein is exposed to water hydrophylic and unstructured then it
usually isn't a problem, but if this isn't the case, it is usually
necessary to have an adapter (if it is possible at all).  Try doing a
hydropathy plot on your protein's AA sequence (if the structure isn't
known).

Michael