plasmid methylation

Robert M. Mihalek mihalek at NOSPAM.smtp.anes.upmc.edu
Wed Sep 24 14:20:20 EST 1997


Is most plasmid DNA fully methylated? In other words, what percent of
plasmids have both strands methylated at every possible Dam+ site
(GATC)? If the percentage is low, can anything be done to increase the
methylation of plasmid DNA?

Many thanks for advice.

Bob Mihalek
University of Pittsburgh
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