barrs01 at doc.mssm.edu
Wed Sep 24 20:44:49 EST 1997
I am having a difficult time subcloning small PCR products (300 and 700
bp) with EcoRI ends into the EcoRI site of the retroviral vector pBABE.
Most of my clones are a recombinant species which is smaller than the
vector itself, the rest are religated vector (inspite of CIP treatment).
Has anyone else had this problem? Do you think it is a ligation problem
or do you think that the recombination is happening on the plate? Anyone
have a suggestion? After 160 miniscreens I'm at my wits' end! Please
reply via e-mail to barrs01 at doc.mssm.edu
Thanks for any help you can offer!
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