Biotin and Streptavidin

Keld Sorensen KeldS at uic.edu
Fri Sep 26 10:34:31 EST 1997


No special requirements - actually this is one of the strongest bonds
know (the dissociation konstant is reported to be 10E-15 ) - the
interaction will usually survive short boiling (100 deg C).

M. Abbott wrote:
> What is the salt requirement(if any) to maintain the interaction between
> biotin and streptavidin?  How temperature sensitive is this interaction?



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