DpnI digestion?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Sep 30 03:26:05 EST 1997


In article <slrn62vthi.1r4.aiyar at ebv.oncology.wisc.edu>, Ashok Aiyar
<aiyar at ebv.oncology.wisc.edu> writes
>An added complication is that recombinant DpnI is purified from 
>dam(-) strains that are endoA(+).  Every preparation of recombinant 
>DpnI that I have purchased from various vendors contains low level of 
>endoA activity.

On a general note do you pick up endoA activity any any other
recombinant RE's? I can't believe they are all produced in endoA(-)
E.coli. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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