pcr on plant genomic

Jon West jpw at mole.bio.cam.ac.uk
Tue Sep 30 10:56:16 EST 1997


Frederik Boernke <boernke at ipk-gatersleben.de> wrote:
>Martin Goodson wrote:
>> 
>> hello all,
>> 
>> I've heard that PCR on plant genomic DNA has specific diffulties not
>> encountered with the DNA of other organisms. Do you know anything about this
>> or other problems in the molecular biology of plants (Reverse transcription
>> etc.)?
>> 
>> martin goodson
>
>Hi Martin,
>
>we have been in plant molecular biologie for quiete a long time and we
>do not feel that there problems with PCR or RT-PCR if using a proper
>protocol.
>
>Cheers 
>Ricky


Me neither mate, try using cDNA from a library with the PCR or contact 
a distant collegue if thats no good :)

Jon





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