Fluorescein labeled dsDNA standard?

Paul N. Hengen pnh at ncifcrf.gov
Tue Sep 30 17:54:14 EST 1997


I've changed detection schemes to fluorescein label.
Now I need a dsDNA standard labeled with fluorescein
and with fragments in the range of 10-200 bp. I found
a pBR322/HinfI marker from Bio-Rad, but the smallest
fragment is 75 bp. Does anyone know of a better one?
Maybe I can cut the HinfI fragments. Any suggestions
as to which enzyme would give me the best spread of
markers in a double digest?

Thanks!

--
Paul N. Hengen, Ph.D.
National Cancer Institute
mailto:pnh at ncifcrf.gov
http://www-lmmb.ncifcrf.gov/~pnh/



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