long-PCR

Andrew Warkman awarkman at julian.uwo.ca
Wed Apr 1 09:32:52 EST 1998


>I have a problem with polymerazing a fragment of at least 15 kb on 
>genomic DNA. Did anyone of you ever managed to amplify 15 kb? Which 
>enzyme did you use?

You may want to look at Tth pol.  PE claims amplification of fragments up to  
~40 kb (if i remember correctly) but i have never used it....

Andy



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