Ligate, blunt ending and ligate
carl.fossdal at nisk.no
Wed Apr 1 07:12:42 EST 1998
Question 1 is:
Have an insert that share one sticky end with the vector and the other end
of the insert and vector must be blunted before ligation.
Wondered if i could first ligate the sticky end, then blunt the two others
before a second ligation. In theory it sound ok? (or should I just blunt
them both first and then ligate?)
Have anyone tried this?
Carl.Fossdal at nisk.no
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