Ligate, blunt ending and ligate

foc carl.fossdal at nisk.no
Wed Apr 1 07:12:42 EST 1998


Hi!

Question 1 is:

Have an insert that share one sticky end with the vector and the other end
of the insert and vector must be blunted before ligation.

Wondered if i could first ligate the sticky end, then blunt the two others
before a second ligation. In theory it sound ok? (or should I just blunt
them both first and then ligate?)

Have anyone tried this?

Carl.Fossdal at nisk.no







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