Blotting from Acrylamide Sequencing Gel

Warren Gallin wgallin at gpu.srv.ualberta.ca
Thu Apr 2 11:38:50 EST 1998


In Article <35236C3B.FE73BF32 at rpms.ac.uk>, mjones at rpms.ac.uk (Dr Mick Jones)
wrote:
>Hi
>
>Has anybody got a protocol or done the following.
>
>Run total genomic DNA on a 12% urea sequencing gel, and then blotted
>(via eletro-transfer) to a nylon membrane, and probe as per Southern.
>
>The aim is to detect DNA fragments that differ by one base pair (in the
>region 100 - 150 bases)
>
>Any thoughts and ideas greatfully received

The Church and Gilbert genomic sequencing procedure does basically this.  It
was published in PNAS in 1984 sometime.

Warren Gallin
Department of Biological Sciences
University of Alberta
Edmonton,  Alberta     T6G 2E9
Canada
wgallin at gpu.srv.ualberta.ca



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