assay for free cysteines

Yu Wai Chen ywc at mrc-lmb.cam.ac.uk
Thu Apr 2 07:02:10 EST 1998


Dear all,

I would like to know if the 6 cysteines of my protein is
disulphide-bridged or not.  Can some one point me to a good reference or
protocol how to achieve this?  I've heard that we can use Ellman's
reagent to do it...  Is Ellman's reagent good for buried cysteines as
well?  Thanks.

-- 
===================================================================
Yu Wai CHEN, Ph.D. ..................  email: ywc at mrc-lmb.cam.ac.uk
 Centre for Protein Engineering,              tel: 44-(1223) 402148
 MRC Centre, Cambridge  CB2 2QH, U.K.         fax: 44-(1223) 402140
 WWW homepage: http://www.mrc-cpe.cam.ac.uk/people/wai.html



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