assay for free cysteines

Yu Wai Chen ywc at
Thu Apr 2 07:02:10 EST 1998

Dear all,

I would like to know if the 6 cysteines of my protein is
disulphide-bridged or not.  Can some one point me to a good reference or
protocol how to achieve this?  I've heard that we can use Ellman's
reagent to do it...  Is Ellman's reagent good for buried cysteines as
well?  Thanks.

Yu Wai CHEN, Ph.D. ..................  email: ywc at
 Centre for Protein Engineering,              tel: 44-(1223) 402148
 MRC Centre, Cambridge  CB2 2QH, U.K.         fax: 44-(1223) 402140
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