Blotting from Acrylamide Sequencing Gel

Dr Mick Jones mjones at
Thu Apr 2 05:46:43 EST 1998


Has anybody got a protocol or done the following.

Run total genomic DNA on a 12% urea sequencing gel, and then blotted
(via eletro-transfer) to a nylon membrane, and probe as per Southern.

The aim is to detect DNA fragments that differ by one base pair (in the
region 100 - 150 bases)

Any thoughts and ideas greatfully received


Mick Jones

Dr M D Jones                      Tel: 081-383-3328 (+44-81-383-3328)
Department of Virology          FAX: 081-383-2299 (+44-81-383-2299)
RPMS,  Du Cane Road              Email: mjones at
London,  W12 0NN,  UK
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"Crackin' toast, Gromit."    Wallace (The Wrong Trousers)

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