PCR Mistake

[ddsyr at PUBLIC.EAST.CN.NET]

Dear Netters:
I have a general question. When PCR a cDNA of known sequence, from same plant species, but different 
cultivars, the sequence of this cDNA may have some different with the publishing sequence. Then can we 
have any way to jugde that some base different are from the Taq enzyme mistakes, and some are from the 
cultivar different?
I am appreciate your suggestion.
Please reply to my email of ddsyr at public.east.cn.net
Ma