long-PCR

Dr. Duncan Clark duncan at genesys.demon.co.uk
Thu Apr 2 03:20:59 EST 1998


In article <6ftj7q$2rq at falcon.ccs.uwo.ca>, Andrew Warkman
<awarkman at julian.uwo.ca> writes
>You may want to look at Tth pol.  PE claims amplification of fragments up to  
>~40 kb (if i remember correctly) but i have never used it....

You will need more than just Tth pol. The PE mix is Tth and a proof-
reading pol using a proprietary reaction buffer. Tth on it's own will
only do a few kb.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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