EcoRI methylation

jheus at pharming.nl jheus at pharming.nl
Fri Apr 3 05:20:06 EST 1998


LS,

Could anyone solve the following dispute:

The EcoRI recognition
sequence can be methylated (CpG) when preceded by a C (CGAATTC) or followed
by a G (GAATTCG). Not clear to me, however, is whether EcoRI is actually
inhibited by this methylation.
Promega and Stratagene catalogs suggest that
it is blocked, while Fermentas and NEB catalogs state that it isn't. Who is
right?

Thanks,  Joris Heus

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