Electroporation of sea urchin eggs

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Fri Apr 3 22:40:51 EST 1998


In article <351DE794.47E at san.rr.com>, bzeller1 at san.rr.com wrote:
>Try looking at our ascidian paper from the Feb. 97 Development.  It
>explains how we electroporate plasmids into fertilized eggs.  I have
>tried S. purpuratus and several other species of sea urchins with no
>luck.  David Epel at Hopkins can use electrporation to load su eggs with
>small molecules but hasn't tried DNA that I know of.  Hope that helps. 
>BZ

So what's the problem? Electroporation works pretty much with everything...
At least as far as getting DNA inside the cell.

        - Dima
 




More information about the Methods mailing list