wgschech at med.uni-tuebingen.de
Sun Apr 5 09:00:15 EST 1998
I've got dishes in the freezer where I had treated cell layers with
triton-x-100 for solubilizing plasma membranes and cytosol.
Now the nuclei are frozen down there and I'm interested in measuring
nuclear enzyme activity (WHAT a *nuclear* energy power this will
unleash!!! ;-) So, SDS will be too heavy for that purpose (of course
good enough for going onto a PAGE gel with them).
What do you suggest instead of scraping them off and sonicate
(SONYcate ?? - using my walkman?? - uuh sorry, that's the sunday lab
Any input is welcome!
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