Methods for quantification of gene expression

ELADER at AMBION.COM ELADER at AMBION.COM
Mon Apr 6 19:06:11 EST 1998


In article <35249c17.9284705 at news.uni-konstanz.de>,
  Simon.Mauch at uni-konstanz.de (Simon Mauch) wrote:

> To come to an end....: If the level of expression of your gene of
> interest is middle to high and if it is no problem to get enough RNA
> from your tissue, I would recommend Northerns. If you want to monitor
> several genes in one assay you might get happy with protection assays.
> And if you intend to study low level expression or if you do have
> problems getting enough RNA from your tissues RT-PCR will be the
> method of choice....

Hey Simon, well said!

Eric

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