Long PCR of genomic DNA

John Fellers jfellers at unity.ncsu.edu
Tue Apr 7 05:48:11 EST 1998


I am trying to amplify up tp 10 kb of genomic DNA from tobacco.  Has
anyone tried to amplify this length, and if so what were your
conditions.  I am currently using a equal ratio of Taq and Pfu plus a
modified 10X buffer.  Any help will be appreciated



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