B12 mutant strain

Ke-Xue Huang khuang at BIOC.RICE.EDU
Wed Apr 8 16:58:35 EST 1998



Are you sure thiamine is vitamin B12?

As I know, thiamine is vitamin B1, by now, nobody can prove any E. coli 
produce vitamin B12.

Huang


On 8 Apr 1998, Dr. Peter Gegenheimer wrote:

> On Fri, 27 Mar 1998 06:59:57, Rich L <lm11 at gpu.srv.ualberta.ca> 
> wrote:
> 
> > Hi netters,
> > 
> > I want to use vitamin B12 mutant E.coli strain for my research, does
> > anybody out there have any information about this? I checked almost every
> > company, but no one sells it.
> > 
> > Any info will be appreciated
> > 
> > Rich
> 
> A large number of E. coli K12 strains are unable to synthesize 
> thiamine (vitamin B12). The relevant genotype is "thi-1" ("thi", 
> "thi-" or "thi1"). Among the commonly-used cloning strains, C600, 
> DH1, DH5-alpha, JM101 and derivatives, NM522, and XL1-Blue and 
> derivatives are thi1, whereas HB101 and derivatives are not 
> (according to the New England Biolabs and Stratagene catalogues). 
> 
> For strains with the exact genotype you need, look at the Web site 
> for the E. coli Genetic Stock Center at Yale University: 
> http://CGSG.biology.yale.edu/ .
> 
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> o
> | Dr. Peter Gegenheimer      | Vox: 785-864-3939  FAX: 785-864-5321 
> |
> | Department of Biochemistry |   PGegen at UKans.nospam.edu            
> |
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